Rapid assembly of phage contigs within YAC clones
نویسندگان
چکیده
منابع مشابه
Method for rapid restriction analysis of YAC clones.
The development of the yeast artificial chromosome (YACs) technology (3) has provided, by virtue of its capacity to carry large fragments of genomic DNA, the possibility of linking the genetic map and the physical map of an organism. This requires the alignment of overlapping YAC clones into contigs for which a number of strategies have been described as follows: the use of sequence tagged site...
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A physical map of rice chromosome 5 was constructed with yeast artificial chromosome (YAC) clones along a high-resolution molecular linkage map carrying 118 DNA markers distributed over 123.7 cM of genomic DNA. YAC clones have been identified by colony and Southern hybridization for 105 restriction fragment length polymorphism (RFLP) markers and by polymerase chain reaction (PCR) screening for ...
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We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 end sequences of P1, TAC and BAC clones. The entire genome of chromosome 3, except for the centrom...
متن کاملA novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.
The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA frag...
متن کاملAssembly of fingerprint contigs: parallelized FPC
SUMMARY One of the more common uses of the program FingerPrint Contigs (FPC) is to assemble random restriction digest 'fingerprints' of overlapping genomic clones into contigs. To improve the rate of assembling contigs from large fingerprint databases we have adapted FPC so that it can be run in parallel on multiple processors and servers. The current version of 'parallelized FPC' has been used...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.10.2795